Espécies Sentinelas: Monitoramento Ambiental com Base em Biomarcadores de Efeito Cito- e Genotóxico
DOI:
https://doi.org/10.37002/revistacepsul.vol12.2333e20230002Palabras clave:
biomarcador, monitoramento, qualidade ambiental, conservação, crustáceo, peixeResumen
O crescimento demográfico humano tem promovido extrema elevação dos impactos antrópicos, acarretando declínio da biodiversidade mundial em vários ecossistemas, tornando imprescindível a aplicação de monitoramentos eficazes e ações efetivas de conservação. Atualmente, existe uma tendência de que os monitoramentos sejam de menor custo operacional e financeiro, bem como que possam ser desenvolvidos por longa duração. Os biomarcadores de efeito (p. ex., ensaios cometa, micronúcleo e vermelho neutro), têm sido empregados com sucesso em espécies emblemáticas, que têm se mostrado um ótimo reflexo da qualidade de um ambiente. Tais espécies, nomeadas "sentinelas ambientais", evidenciam impactos antrópicos danosos ainda nos momentos iniciais, antecipando ações de gestão que evitem danos persistentes (Ã s vezes irreversíveis) nas espécies, populações e comunidades locais, que somente seriam constatados futuramente. Espécies sentinelas precisam ser resistentes o suficiente, residentes do ambiente em avaliação, possuir reduzida vagilidade e mostrar interação com o maior número de componentes ambientais, sejam eles abióticos ou bióticos. Os trabalhos desenvolvidos com espécies de invertebrados (p. ex., bivalves e crustáceos) e vertebrados (p. ex., peixes e mamíferos) têm mostrado a excelência e eficácia dos biomarcadores, com avaliações no nível celular e molecular. O presente estudo visa apresentar os procedimentos que devem ser adotados durante a avaliação da qualidade ambiental pelo uso de biomarcadores de efeito (genotoxicidade e citotoxicidade), desde a escolha das espécies sentinelas, os cuidados na condução dos protocolos e a eficácia dos métodos em casos reais.
Citas
ADAM, M. L., DE ASSIS ROCHA PESSOA, L., DE LIMA, A. R. B. & BERNARD, E. 2022. DNA damage as indicator of the environmental vulnerability of bats in Brazil's Caatinga drylands. Environ. Monit. Assess., 194(4): 1-17.
ADAM, M. L., TORRES, R. A., SPONCHIADO, G., MOTTA, T. S., OLIVEIRA, C. M. R., CARVALHO-FILHO, M. A. & CORREIA, M. T. S. 2010. Environmental degradation at a park in southern Brazil as revealed through a genotoxicity test (MN) on peripheral blood cells from Poecilia vivipara (Teleostei). Water Air & Soil Pollut., 211: 61-68.
AMADI, C. N., FRAZZOLI, C. & ORISAKWE, O. E. 2022. Sentinel species for biomonitoring and biosurveillance of environmental heavy metals in Nigeria. J. Environ. Sci. Health, Part C, 38(1): 21-60.
AGUIRRE-MARTNEZ, G. V., BURRATI, S., FABRI, E., DEL VALLS, T. A. & MARTIN-DIAZ, M. L. 2013. Stability of lysosomal membrane in Carcinus maenas acts as biomarker of exposure to pharmaceuticals. Environ. Monit. Assess., 185(5): 3783-3793.
ARANTES, A. C. R., ADAM, M. L., SOUZA, J. R. B., BASTOS, L. P., JACOBINA, U. P. & TORRES, R. A. 2016. Frequency of fish micronuclei to diagnose aquatic environmental conditions from Brazilian megacities: a case study of Iguaçu river, Southern Brazil. Rev. Bras. Biociências, 14(2).
ARECES, A. J., COCENTINO, A. L. M., REIS, T. N. V., VASCONCELOS, E. R. T. P. P., GUIMARÃES-BARROS, N. C., FUJII, M. T. 2015. Las Macroalgas como Bioindicadoras da Calidad Ambiental y Cambios Climaticos. Guia pratica. Braz. J. Ecol., 1-64: 1516-5868.
ASTLEY, K. N., MEIGH, H. C., GLEGG, G. A., BRAVEN, J. & DEPLEDGE, M. H. 1999. Multivariate analysis of biomarker responses in Mytilus edulis and Carcinus maenas from the Tees Estuary (UK). Mar. Pollut. Bull., 39(1-2): 145-154.
BADALOTTI, A., VAN GALEN. L., VIÉ, J. C. & STEPHENSON, P. J. 2022. Improving the monitoring of conservation programs: lessons from a grant-making initiative for threatened species. Oryx, 56: 288-294.
BAROUDI, F., AL ALAM, J., FAJLOUN, Z. & MILLET, M. 2020. Snail as sentinel organism for monitoring the environmental pollution; a review. Ecol. Indic., 113: 106240.
BEEVER, J. W., SIMBERLOFF-III, D. & KING, L. L. 1979. Herbivory and predation by the mangrove tree crab Aratus pisonii. Oecol., 43: 317-328.
BHAT, S. A., CUI, G., LI, F. & VIG, A. P. 2019. Biomonitoring of genotoxicity of industrial wastes using plant bioassays. Biores. Tech. Rep., 6: 207-216.
BOLOGNESI, C. & CIRILLO, S. 2014. Genotoxicity biomarkers in aquatic bioindicators. Curr. Zool., 60(2): 273-284.
BUENFIL-ROJAS, A. M., ALVAREZ- LEGORRETA, T., GONZÁLEZ-JÁUREGUI, M., RENDÓN-VON OSTEN, J. & CEDEÑO-VAZQUEZ, J. R. 2022. Effectiveness of Morelet's crocodile as a bioindicator of metal pollution and metallothionein response to spatial variations of metal exposure. Environ. Adv., 100251.
CARDONA, L., CAMPOS, N. H. & ROLONG, E. H. 2019. Parámetros de crecimiento de Cardisoma guanhumi en el departamento del Magdalena, Colombia. Bull. Mar. Coast. Res., 48(1): 27-41.
CARRASCO, K. R., TILBURY, K. L. & MYERS, M. S. 1990. Assessment of the piscine micronucleus test as an in situ biological indicator of chemical contaminant effects. Can. J. Fish. Aquat. Sci., 47(11): 2123-2136.
CASTILHOS-GHISI, N., LARENTIS, C., OLIVEIRA, E. C., NEVES, M. P., ZAVASKI, A. G., ALMEIDA-ROQUE, A., WACHTEL, C. C., SILVA, A. P., LIMA, E. B. S., COSTA, G. O. N., AMORIM, J. P. A., RIBEIRO, C. A. O., DELARIVA, R. L. 2022. Environmental assessment of Neotropical streams using fish as bioindicators: a multibiomarker and integrated approach. Hydrobiologia, 849: 4587-4604.
CHELLIAN, R., PANDY, V. & MOHAMED, Z. 2017. Pharmacology and toxicology of α-and β-Asarone: A review of preclinical evidence. Phytomed., 32: 41-58.
CHRISTOFOLETTI, R. A., HATTORI, G. Y. & PINHEIRO, M. A. A. 2013.Food selection by a mangrove crab: temporal changes in fasted animals. Hydrobiol., 702: 63-72.
COLIN, N., PORTE, C., FERNANDES, D., BARATA, C., PADRÓS, F., CARRASSÓN, M., ... & MACEDA-VEIGA, A. 2016. Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers. Sci. Total Environ., 540: 307-323.
CONSTABLE, A. J., COSTA, D. P., SCHOFIELD, O., NEWMAN, L., URBAN JR, E. R., FULTON, E. A., ... & WILLIS, Z. 2016. Developing priority variables ("ecosystem Essential Ocean Variables"—eEOVs) for observing dynamics and change in Southern Ocean ecosystems. J. Mar. Syst., 161: 26-41.
CORDIER, T., ALONSO-SAEZ, L., APOTHELOZ-PERRET-GENTIL, L., AYLAGAS, E., BOHAN, D. A., BOUCHEZ, A., CHARITON, A., CREER, S., FRÃœHE, L., KECK, F., KEELEY, N., LAROCHE, O., LEESE, F., POCHON, X., STOECK, T., PAWLOWSKI, J. & LANZEN, A. 2020. Ecosystems monitoring powered by environmental genomics: A review of current strategies with an implementation roadmap. Mol. Ecol., 46: 235-248.
CUNNINGHAM, M. M., TRAN, L., MCKEE, C. G., POLO, R. O., NEWMAN, T., LANSING, L., ... & GUARNA, M. M. 2022. Honey bees as biomonitors of environmental contaminants, pathogens, and climate change. Ecol. Indic., 134: 108457.
DA SILVA, D. M. 2021. Diagnóstico e monitoramento do rio Goiana (PE-Brasil), por parâmetros de danificação genômica, após acidente ambiental. Recife. 97p. (Dissertação de Mestrado. Programa de Pós-Graduação em Biologia Animal. Universidade Federal de Pernambuco).
DA SILVA, L. F., NOBRE, C. R., MORENO, B. B., PEREIRA, C. D. S., DE SOUZA ABESSA, D. M., CHOUERI, R. B., ... & CESAR, A. 2022. Non-destructive biomarkers can reveal effects of the association of microplastics and pharmaceuticals or personal care products. Mar. Pollut. Bull., 177: 113469.
DAILIANIS, S., DOMOUHTSIDOU, G. P., RAFTOPOULOU, E., KALOYIANNI, M. & DIMITRIADIS, V. K. 2003. Evaluation of neutral red retention assay, micronucleus test, acetylcholinesterase activity and a signal transduction molecule (cAMP) in tissues of Mytilus galloprovincialis (L.), in pollution monitoring. Mar. Environ. Res., 56: 443-470.
DE LA TORRE, F. R., SALIBIÁN, A. & FERRARI, L. 2007. Assessment of the pollution impact on biomarkers of effect of a freshwater fish. Chemosphere, 68(8): 1582-1590.
DE LIMA E SILVA, J. R., DE LIMA, A. R. B., DA SILVA, D. L., ROSA FILHO, J. S. & ADAM, M. L. 2022. Contrasting tourism regimes due to the COVID-19 lockdown reveal varied genomic toxicity in a tropical beach in the Southern Atlantic. Environ. Monit. Assess., 194(7): 1-19.
DE SOUZA, P. R., DE SOUZA, K. S., DE ASSIS, C. R. D., DE ARAÚJO, M. C., SILVA, K. C. C, DA SILVA, J. D. F. X., ... & DE SOUZA BEZERRA, R. 2018. Acetylcholinesterase of mangrove oyster Crassostrea rhizophorae: A highly thermostable enzyme with promising features for estuarine biomonitoring. Aquat. Toxicol., 197: 109-121.
DOS SANTOS, G. P. C., DE ASSIS, C. R. D., OLIVEIRA, V. M., CAHU, T. B., SILVA, V. L., SANTOS, J. F., ... & BEZERRA, R. S. 2022. Acetylcholinesterase from the charru mussel Mytella charruana: kinetic characterization, physicochemical properties and potential as in vitro biomarker in environmental monitoring of mollusk extraction areas. Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol., 252: 109225.
DUARTE, L. F. A., SOUZA, C. A., NOBRE, C. R., PEREIRA, C. D. S. & PINHEIRO, M. A. A. 2016. Multi-level biological responses in Ucides cordatus (Linnaeus, 1763) (Brachyura, Ucididae), as indicators of conservation status in mangrove areas from the Western Atlantic. Ecotoxicol. Environ. Saf., 133: 176-187.
DUARTE, L. F. A., SOUZA, C. A., PEREIRA, C. D. S. & PINHEIRO, M. A. A. 2017. Metal toxicity assessment by sentinel species of mangroves: In situ case study integrating chemical and biomarkers analyses. Ecotoxicol. Environ. Saf., 145: 367-376.
ESTES, J. A., HEITHAUS, M., MCCAULEY, D. J., RASHER, D. B. & WORM, B. 2016. Megafaunal impacts on structure and function of ocean ecosystems. Ann. Rev. Environ. Res., 41(1): 83-116.
FALCÃO, C. B. R., PINHEIRO, M. A. A., TORRES, R. A. & ADAM, M. L. 2020. Spatial-temporal genome damaging in the blue crab Cardisoma guanhumi as ecological indicators for monitoring tropical estuaries. Mar. Pollut. Bull., 156: 111232.
FENECH, M. 2000. The in vitro micronucleus technique. Mut. Res./Fund. Mol. Mech. Mutagen., 455(1-2): 81-95.
FLEMING, A. H., CLARK, C. T., CALAMBOKIDIS, J. & BARLOW, J. 2016. Humpback whale diets respond to variance in ocean climate and ecosystem conditions in the California Current. Glob. Change Biol., 22: 1214-24.
GAD, S.C. 2014. Glutathione. In: Encyclopedia of Toxicology. Elsevier, p. 751.
GATISO, T. T., KULIK, L., BACHMANN, M., BONN, A., BÖSCH, L., FREYTAG, A., HEURICH, M., WESCHE, K., WINTER, M., ORDAZ-NÉMETH, I., SOP. T. & KÃœHL, H. S. 2022. Sustainable protected areas: Synergies between biodiversity conservation and socioeconomic development. People and Nature, 4(4): 893-903.
GONZALEZ-HUNT, C. P., WADHWA, M. & SANDERS, L. H. 2018. DNA damage by oxidative stress: Measurement strategies for two genomes. Curr. Opinion Toxicol., 7: 87-94.
GÃœRKAN, S. E. 2022. Impact of Nickel Oxide Nanoparticles (NiO) on Oxidative Stress Biomarkers and Hemocyte Counts of Mytilus galloprovincialis. Biol. Trace Element Res., 1-13.
HARCOURT, R., SEQUEIRA, A. M., ZHANG, X., ROQUET, F., KOMATSU, K., HEUPEL, M., ... & FEDAK, M. A. 2019. Animal-borne telemetry: an integral component of the ocean observing toolkit. Front. Marine Sci. 6: 326.
HAUTON, C., HAWKINS, L. E. & HUTCHINSON, S. 1998. The use of neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L). Comp. Biochem. Physiol. B, 119: 619-623.
HAZEN, E. L., ABRAHMS, B., BRODIE, S., CARROLL, G., JACOX, M. G., SAVOCA, M. S., ... & BOGRAD, S. J. 2019. Marine top predators as climate and ecosystem sentinels. Front. Ecol. Environ., 17(10): 565-574.
HO, C., ROBINSON, A., MILLER, D. & DAVIS, M. 2005. Overview of Sensors and Needs for Environmental Monitoring. Sensors, 5: 4-37.
IMAM, R. S. 2019. Genotoxicity of monosodium glutamate: A review on its causes, consequences and prevention. Indian J. Pharmaceut. Educ. Resear., 53(4s): S510-517.
JETZ, W., TERTITSKI, G., KAYS, R., MUELLER, U., WIKELSKI, M., AKESSON, S., ... & RAKHIMBERDIEV, E. 2022. Biological Earth observation with animal sensors. Trends Ecol. Evol., 37(4): 293-298.
KHAN, A., SICEN, L., KHAN, B. & SALMAN, N. 2021. On the influence of demographic structure and industrial growth on environmental quality. J. Environ. Manag., 288: 112453.
KIENZL, K., RISS, A., VOGEL, W., HACKL, J. & GOTZ, B. 2003. Bioindicators and biomonitors for policy, legislation and administration, In: MARKERT, B. A., BREURE, A. M. & ZECHMEISTER, H. G. (eds.). Trace Metals and other Contaminants in the Environment. Elsevier, Chap 3: 85-122.
KING, M. A. 2000. Detection of dead cells and measurement of cell killing by flow cytometry. J. Immunol. Meth., 243: 155-166.
KOAGOUW, W. & CIOCAN, C. 2018. Impact of metformin and increased temperature on blue mussels Mytilus edulis-evidence for synergism. J. Shellfish Res., 37(3): 467-474.
LAM, P. K. 2009. Use of biomarkers in environmental monitoring. Ocean Coast. Manag., 52(7): 348-354.
LEE, R.F. & STEINERT, S. 2003. Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mut. Res./Rev. Mut. Res., 544(1): 43-64.
LEITE, D. S. L., RIUL, P., DE FREITAS, N. D. A. & DE MIRANDA, G. E. C. 2020. Evaluation of the conservation status and monitoring proposal for the coastal reefs of Paraíba, Brazil: Bioindication as an environmental management tool. Ocean Coast. Manag., 194: 105208.
LEUNG, H. M., SUNG, K. C., YUE, P. Y. K., PENG, X. L., CHEUNG, K. C., AU, C. K., ... & LI, W. C. 2022. Characterization of Maize Cytochrome P450 Monooxygenases Induced in Response to 2,3,7,8 Tetrachlorodibenzodioxin: The Role of Indigenous Biomarkers in Environmental Monitoring of Contaminated Sites. PREPRINT (Version1) available at Research Square, 1-10. <https://doi.org/10.21203/rs.3.rs-1164167/v1>.
LIMA, A. R., TORRES, R. A., JACOBINA, U. P., PINHEIRO, M. A. A. & ADAM, M. L. 2019. Genomic damage in Mugil curema (Actinopterygii: Mugilidae) reveals the effects of intense urbanization on estuaries in northeastern Brazil. Mar. Pollut. Bull, 138: 63-69.
LIONETTO, M. G., CARICATO, R. & GIORDANO, M. E. 2021. Pollution biomarkers in the framework of marine biodiversity conservation: State of art and perspectives. Water, 13(13): 1847.
LOWE, D. M. & PIPE, R. K. 1994. Contaminant induced lysosomal membrane damage in marine mussel digestive cells: an in vitro study. Aquat. Toxicol., 30: 357-365.
LUNDQUIST, C. J. & GRANEK, E. F. 2005. Strategies for Successful Marine Conservation: Integrating Socioeconomic, Political, and Scientific Factors. Conserv. Biol., 19: 1771-1778.
MAGNUSSON, W. E. 2014. Uncertainty and the design of in-situ biodiversity-monitoring programs. Nat. Conserv., 8: 77-94.
MARTIÌNEZ-GOÌMEZ, C., BENEDICTO, J., CAMPILLOA, J. A. & MOORE, M. 2008. Application and evaluation of the neutral red retention (NRR) assay for lysosomal stability in mussel populations along the Iberian Mediterranean coast. J. Environ. Monit., 10: 490-499.
MAXWELL, S. L., CAZALIS, V., DUDLEY, N., HOFFMANN, M., RODRIGUES, A. S. L., STOLTON, S., VISCONTI, P., WOODLEY, S., KINGSTON, N., LEWIS, E., MARON, M., STRASSBURG, B. B. N., WENGER, A., JONAS, H. D., VENTER, O. & WATSON, J. E. M. 2020. Area-based conservation in the twenty-first century. Nature, 586: 217-227.
MAYER, F. L., VERSTEEG, D. J., MCKEE, M. J., FOLMAR, L. C., GRANEY, R. L., MCCUME, D. C. & RATTNER, B. A. 1992. Physiological and nonspecific biomarkers. In: HUGGETT, R. J., KIMERLE, R. A., MEHRLE, J. R. P. M. & BERGMAN, H. L. (eds.). Biomarkers: Biochemical, Physiological, and Histological Markers of Anthropogenic Stress. Lewis, Boca Raton, FL, USA, 125-153.
MEDEIROS, L. C. C., DELUNARDO, F. A. C., SIMÕES, L. N., PAULINO, M. G., VARGAS, T. S., FERNANDES, M. N., ... & CHIPPARI-GOMES, A. R. 2017. Water-soluble fraction of petroleum induces genotoxicity and morphological effects in fat snook (Centropomus parallelus). Ecotoxicol. Environ. Saf., 144: 275-282.
MILOSLAVICH, P., BAX, N. J., SIMMONS, S. E., KLEIN, E., APPELTANS, W., ABURTO-OROPEZA, O., ANDERSEN-GARCIA, M., BATTEN, S. D., BENEDETTI-CECCHI, L., CHECKLEY, D. M., CHIBA, S., DUFFY, J. E., DUNN, D. C., FISCHER, A., GUNN, J., KUDELA, R., MARSAC, F., MULLER-KARGER, F. E., OBURA, D. & SHIN, Y. 2018. Essential ocean variables for global sustained observations of biodiversity and ecosystem changes. Global Change Biol., 24: 2416-2433.
MISHRA, B., KUMAR, P., SARASWAT, C., CHAKRABORTY, S. & GAUTAM, A. 2021. Water Security in a Changing Environment: Concept, Challenges and Solutions. Water, 13, 490.
MMA. 2022. Cadastro Nacional de Unidades de Conservação (CNUC). Disponível em: <https://cnuc.mma.gov.br>. Acesso em: 28 set. de 2022.
MONCALEANO-NIÑO, A. M., GÓMEZ-CUBILLOS, M. C., LUNA-ACOSTA, A., VILLAMIL, L., CASSERES-RUIZ, S. & AHRENS, M. J. 2022. Monitoring metallothionein-like protein concentrations and cholinesterase activity in tropical cup oysters as biomarkers of exposure to metals and pesticides in the southern Caribbean, Colombia. Environ. Sci. Pollut. Res., 29(17): 25157-25183.
MOORE, M. N. 1985. Cellular response to pollutants. Mar. Pollut. Bull.,16: 134-139.
NAGARANI, N., KRISHNAVENI, G., DHARSHINI, V. D., MANOHARI, K. G., JEYAPANDI, M. A., MARIAPPAN, P. V., SANGEETHA, R. 2023. Biomarkers as ecological indices in monitoring the status of market fish. The Journal of Basic and Applied Zoology, 84: 2-6.
NG, P. K. L., GUINOT, D. & DAVIE, P. J. 2008. Systema Brachyurorum: Part I. An annotated checklist of extant brachyuran crabs of the world. The Raffles Bull. Zool., 17: 1-286.
NICHOLS, J. & WILLIAMS, B. 2006. Monitoring for conservation. Trends Ecol. Evol., 21: 668-673.
OLIVEIRA, L. M. & RODRIGUES, J. J. 2011. Wireless Sensor Networks: a Survey on Environmental Monitoring. JCM, 6: 143-151.
OROZCO-HERNÁNDEZ, J. M., GÓMEZ-OLIVÁN, L. M., ELIZALDE-VELÁZQUEZ, G. A., HEREDIA-GARCÍA, G., CARDOSO-VERA, J. D., DUBLÁN-GARCÍA, O., ISLAS-FLORES, H., SANJUAN-REYES, N. & GALAR-MARTÍNEZ, M. 2022. Effects of oxidative stress induced by environmental relevant concentrations of fluoxetine on the embryonic development on Danio rerio. Sci. Total Environ., 807: 151048.
PAQUIN, P. R., GORSUCH, J. W., APTE, S., BATLEY, G. E., BOWLES, K. C., CAMPBELL, P. G. C., DELOS, C. G., DI TORO, D. M., DWYER, R. L., GALVEZ, F., GENSEMER, R. W., GOSS, G. G., HOGSTRAND, C., JANSSEN, C. R., MCGEER, J. C., NADDY, R. B., PLAYLE, R. C., SANTORE, R. C., SCHNEIDER, U., STUBBLEFIELD, W. A., WOOD, C. M. & WU, K. B. 2002. The biotic ligand model: a historical overview. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 133: 3-35.
PARRA, S., VARANDAS, S., SANTOS, D., FÉLIX, L., FERNANDES, L., CABECINHA, E., GAGO, J. & MONTEIRO, S. M. 2021. Multi-biomarker responses of Asian clam Corbicula fluminea (Bivalvia, Corbiculidea) to cadmium and microplastics pollutants. Water, 13: 394.
PEREIRA, C. D. S., ABESSA, D. M. S., BAINY, A. C., ZARONI, L. P., GASPARRO, M. R., BIÌCEGO, M. C., TANIGUCHI, S., FURLEY, T. H. & MOREIRA DE SOUSA, E. C. P. 2007. Integrated assessment of multilevel biomarker responses and chemical analysis in mussels from São Sebastião, São Paulo, Brazil. Environ. Toxicol. Chem., 26(3): 462-469.
PINHEIRO, M. A. A. 2012. Projeto Uçá III - Impacto genotóxico em populações de caranguejo-uçaÌ, Ucides cordatus (Linnaeus, 1763) (Crustacea, Brachyura, Ucididae): Avaliação e correlação com a concentração de metais pesados em seis manguezais do Estado de São Paulo. São Vicente: UNESP / Campus Experimental do Litoral Paulista (CLP) - Relatório Científico Final FAPESP (Proc. nº 2009/14725-1), 153p.
PINHEIRO, M. A. A., DUARTE, L. F. A., TOLEDO, T. R., ADAM, M. L. & TORRES, R. A. 2013. Habitat monitoring and genotoxicity in Ucides cordatus (Crustacea: Ucididae), as tools to manage a mangrove reserve in southeastern Brazil. Environ. Monit. Assess., 185(10): 8273-8285.
PINHEIRO, M. A. A., FISCARELLI, A. G. & HATTORI, G. Y. 2005. Growth of the mangrove crab Ucides cordatus (Brachyura, Ocypodidae). J. Crust. Biol., 25(2): 293-301.
PINHEIRO, M. A. A., KRIEGLER, N., SOUZA, C. A. & DUARTE, L. F. A. 2021. Feeding habit and lifestyle influence the baseline micronuclei frequency of crab species in pristine mangroves. Wetlands, 41: 30. <https://doi.org/10.1007/s13157-021-01427-4>.
PINHEIRO, M. A. A., SOUZA, C. A., ZANOTTO, F. P., TORRES, R. A. & PEREIRA, C. D. S. 2017. The crab Ucides cordatus (Malacostraca, Decapoda, Brachyura) and other related taxa as environmental sentinels for assessment and monitoring of tropical mangroves from South America. In: LARRAMENDI, M. L. (ed.). Ecotoxicology and Genotoxicology Non-traditional Aquatic Models. Issues in Toxicology nº 33. London, Royal Society of Chemistry (RSC), chap. 10: 212-241.
RANK, J., LEHTONEN, K. K., STRAND, J., LAURSEN, M. 2007. DNA damage, acetylcholinesterase activity and lysosomal stability in native and transplanted mussels (Mytilus edulis) in areas close to coastal chemical dumping sites in Denmark. Aquat. Toxicol., 84: 50-61.
RATHORE, S., VARSHNEY, A., MOHAN, S. & DAHIYA, P. 2022. An innovative approach of bioremediation in enzymatic degradation of xenobiotics. Biotech. Gen. Eng. Rev., 38(1): 1-32.
RODRIGUES, A. P. D. C., MACIEL, P. O., DA SILVA, L. P., ALBUQUERQUE, C., INÁCIO, A. F., FREIRE, M., LINDE, A. R., ALMOSNY, N. R. P., ANDREATA, J. V., BIDONE, E. D. & CASTILHOS, Z. C. 2010. Biomarkers for mercury exposure in tropical estuarine fish. J. Braz. Soc. Ecotoxicol., 5(1): 9-18.
ROJAS, E., LOPEZ, M. C. & VALVERDE, M. 1999. Single cell gel electrophoresis assay: methodology and applications. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 722(1-2): 225-254.
SARGSYAN, A., SIMONYAN, A., HOVHANNISYAN, G., ARAKELYAN, M. & AROUTIOUNIAN, R. 2019. Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution. Mut. Res./Gen. Toxicol. Environ. Mutagen., 842: 117-124.
SCALON, M. C. S., RECHENMACHER, C., SIEBEL, A. M., KAYSER, M. L., RODRIGUES, M. T., MALUF, S. W., ... & SILVA, L. B. D. 2010. Evaluation of Sinos River water genotoxicity using the comet assay in fish. Braz. J. Biol., 70: 1217-1222.
SCHLENK, D. 1999. Necessity of defining biomarkers for use in ecological risk assessments. Mar. Pollut. Bull., 39: 48-53.
SEDEÑO-DÍAZ, J. E. & LÓPEZ-LÓPEZ, E. 2012. Fresh water fish as sentinel organism: from the molecular to the population level, a Review. In TURKER, H. (ed.). New advances and contributions to fish biology. InTech. Chap.: 4. .
SERGIO, F., CARO, T., BROWN, D., CLUCAS, B., HUNTER, J., KETCHUM, J., MCHUGH, K. & HIRALDO, F. 2008. Top predators as conservation tools: ecological rationale, assumptions, and efficacy. Ann. Rev. Ecol. Evol. System., 39: 1-19.
SFORZINI, S., MOORE, M. N., OLIVERI, C., VOLTA, A., JHA, A., BANNI, M. & VIARENGO, A. 2018. Role of mTOR in autophagic and lysosomal reactions to environmental stressors in molluscs. Aquat. Toxicol., 195:114-128.
SHARMA, M., CHADHA, P. & SHARMA, P. C. R. 2021. Environmental biomonitoring using different biomarkers and their contribution in aquatic environmental research. Inter. J. Fish. Aquat. Studies, 9(5): 205-214.
SILVA, C. C. & CRUZ, A. D. 2012. Genotoxicidade da triancinolona e do nitrato de prata em linfócitos utilizando o teste cometa. Estudos, 39(2): 155-163.
SILVEIRA, E. D. R., BENVINDO-SOUZA, M., ASSIS, R. A., SANTOS, C. G. A., LIMA-AMORIM, N. P., BORGES, R. E., MELO, C. & SOUZA-SANTOS, L. R. 2022. Micronucleus and different nuclear abnormalities in wild birds in the Cerrado, Brazil. Environ. Sci. Pollut. Res., 29: 14279-14287.
SOMMER, S., BURACZEWSKA, I. & KRUSZEWSKI, M. 2020. Micronucleus assay: the state of art, and future directions. Inter. J. Mol. Sci., 21(4): 1534.
SOUTO, E. B., CAMPOS, J. R., DA ANA, R., MARTINS-GOMES, C., SILVA, A. M., SOUTO, S. B., LUCARINI, M., DURAZZO, A. & SANTINI, A. 2020. Ocular cell lines and genotoxicity assessment. Int. J. Environ. Res. Health, 17(6): 2046.
SOUZA, C. A., DUARTE, L. F. A., ZANOTTO, F. P., ORTEGA, P., MOREIRA, R. G. & PINHEIRO, M. A. A. 2022. Seasonal effect on biomarker responses in sentinel species: innovation in mangrove conservation status assessment. Environ. Monit. Assess., 194: 425.
SPEIT, G. & HARTMANN, A. 1999. The Comet Assay (Single-Cell Gel Test), 203-2012. In: HENDERSON, D. S. (ed.). DNA Repair Protocols. Methods in Molecular Biology. New Jersey: Humana Press, 113: 642p.
SPONCHIADO, G., ADAM, M. L., SILVA, C. D., SOLEY, B. S., DE MELLO-SAMPAYO, C., CABRINI, D. A., CORRER, C. J. & OTUKI, M. F. 2016. Quantitative genotoxicity assays for analysis of medicinal plants: A systematic review. J. Ethnopharmacol., 178: 289-296.
SRINIVAS, U. S., TAN, B. W., VELLAYAPPAN, B. A. & JEYASEKHARAN, A. D. 2019. ROS and the DNA damage response in cancer. Redox Biol., 25: 101084.
STANKEVIČIŪTĖ, M., JAKUBOWSKA, M., PAŽUSIENĖ, J., MAKARAS, T., OTREMBA, Z., URBAN-MALINGA, B., FEY, D. P., GRESZKIEWICZ, M., SAULIUTĖ, G., BARŠIENĖ, J. & ANDRULEWICZ. E. 2019. Genotoxic and cytotoxic effects of 50 Hz 1 mT electromagnetic field on larval rainbow trout (Oncorhynchus mykiss), Baltic clam (Limecola balthica) and common ragworm (Hediste diversicolor). Aquat. Toxicol., 208: 109-117.
STEPHENS, A. D., LIU, P. Z., BANIGAN, E. J., ALMASSALHA, L. M., BACKMAN, V., ADAM, S. A., GOLDMAN, R. D. & MARKO, J. F. 2018. Chromatin histone modifications and rigidity affect nuclear morphology independent of lamins. Mol. Biol. Cell, 29(2): 220-233.
SVENDSEN, C. 2000. Earthworm biomarkers in terrestrial ecosystems. Reading, UK. (Ph.D. Thesis, University of Reading).
SVENDSEN, C., SPURGEON, D. J., HANKARD, P. K. & WEEKS, J. M. 2004. A review of lysosomal membrane stability measured by neutral red retention: is it a workable earthworm biomarker? Ecotoxicol. Environ. Saf., 57(1): 20-29.
SYDEMAN, W. J., POLOCZANSKA, E., REED, T. E. & THOMPSON, S. A. 2015. Climate change and marine vertebrates. Sci., 350: 772-777.
ULLO, S. L. & SINHA, G. R. 2020. Advances in Smart Environment Monitoring Systems Using IoT and Sensors. Sensors, 20: 3113.
VIGNARDI, C. P., HASUE, F. M., SARTÓRIO, P. V., CARDOSO, C. M., MACHADO, A. S., PASSOS, M. J. A. C. R., SANTOS, T. C. A., NUCCI, J. M., HEWER, T. L. R., WATANABE, I. S., GOMES, V. & PHAN, N. V. 2015. Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766). Aquat. Toxicol., 158: 218-229.
VIMAL, R., NAVARRO, L. M., JONES, Y., WOLF, F., LE-MOGUÉDEC, G. & RÉJOU-MÉCHAIN, M. 2021. The global distribution of protected areas management strategies and their complementarity for biodiversity conservation. Biol. Conserv., 256: 109014.
WEDDERBURN, J., CHEUNG, V., BAMBER, S., BLOXHAM, M. & DEPLEDGE, M. H. 1998. Biomarkers of biochemical and cellular stress in Carcinus maenas: an in situ field study. Mar. Environ. Res., 46(1-5): 321-324.
WEEKS, J. M. & SVENDSEN, C. 1996. Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: a simple biomarker of exposure to soil copper. Environ. Toxicol. Chem., 15: 1801-1805.
ZACHARIAS, M. A. & ROFF, J. C. 2001. Use of focal species in marine conservation and management: a review and critique. Aquat. Conserv. Mar. Freshw. Ecosyst., 11(1): 59-76.
ZENG, Y, KOH, L. P. & WILCOVE, D. S. 2022. Gains in biodiversity conservation and ecosystem services from the expansion of the planet's protected areas. Sci. Adv., 8: eabl9885.
Descargas
Archivos adicionales
Publicado
Número
Sección
Licencia
Derechos de autor 2023 Revista CEPSUL - Biodiversidade e Conservação Marinha
![Creative Commons License](http://i.creativecommons.org/l/by/4.0/88x31.png)
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.