Exposición Crónica al Insecticida Piretroide Deltametrina: Implicaciones Hepáticas y Energéticas en Murciélago Frugívoro Artibeus lituratus
DOI:
https://doi.org/10.37002/biobrasil.v13i2.2370Palabras clave:
Estrés oxidativo, Metabolismo energético, GlucosaResumen
Los insecticidas piretroides se han incorporado al mercado como una alternativa menos tóxica para los animales no objetivo. Sin embargo, se sabe que la deltametrina, un insecticida de esta clase, causa toxicidad por exposición aguda y crónica en diferentes animales. Los murciélagos son animales no objetivo que están constantemente expuestos a pesticidas mientras se alimentan y esto puede afectar su supervivencia. El objetivo fue evaluar el efecto de la exposición crónica a deltametrina sobre el estado redox y el metabolismo energético de murciélagos frugívoros de la especie Artibeus lituratus. Para ello, se recolectaron los murciélagos (n=12) y se separaron en: grupo control (Control; n=6), alimentados con frutos sin tratar; Grupo de deltametrina (DM; n = 6),
alimentado con frutos tratados con deltametrina a una concentración de 1,00 mL/L más aplicador adhesivo (0,015 g/L). Después de un período de exposición crónica (35 días), se analizó el estado redox del hígado y el riñón, los cambios histológicos en el hígado, la concentración de glucosa y las reservas de energía. La exposición a dosis comerciales de deltametrina indujo estrés oxidativo y cambios morfológicos en el hígado, además de causar hipoglucemia y reducir las reservas de energía
en murciélagos. Teniendo en cuenta que los murciélagos están naturalmente expuestos a pesticidas durante la búsqueda de alimento, el impacto de esta exposición puede alterar las reservas de energía e inducir cambios en el funcionamiento normal del hígado, perjudicando la supervivencia de estos animales y, en consecuencia, los servicios ecosistémicos que brindan.
Citas
Aebi, H. (1984). [13] Catalase in vitro. In Methods in enzymology (Vol. 105, pp. 121-126). Academic press.
Agrawal, A. N. J. U., & Sharma, B. (2010). Pesticides induced oxidative stress in mammalian systems. Int J Biol Med Res, 1(3), 90-104.
Amaral, T. S., Carvalho, T. F., Silva, M. C., Barros, M. S., Picanço, M. C., Neves, C. A., & Freitas, M. B. (2012). Short-term effects of a spinosyn's family insecticide on energy metabolism and liver morphology in frugivorous bats Artibeus lituratus (Olfers, 1818). Brazilian Journal of Biology, 72, 299-304.
Amin, K. A., & Hashem, K. S. (2012). Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. BMC veterinary research, 8(1), 1-8.
Barbosa, K. B. F., Costa, N. M. B., Alfenas, R. D. C. G., De Paula, S. O., Minim, V. P. R., & Bressan, J. (2010). Estresse oxidativo: conceito, implicações e fatores modulatórios. Revista de nutrição, 23, 629-643.
Ben-Hamo, M., Muñoz-Garcia, A., & Pinshow, B. (2012). Respostas fisiológicas ao jejum em morcegos. Em Fisiologia comparativa do jejum, fome e limitação alimentar (pp. 257-275). Springer, Berlim, Heidelberg.
Bennett, B. S., & Thies, M. L. (2007). Organochlorine pesticide residues in guano of Brazilian free-tailed bats, Tadarida brasiliensis Saint-Hilaire, from East Texas. Bulletin of environmental contamination and toxicology, 78(3), 191-194.
Bianconi, G. V., Mikich, S. B., Teixeira, S. D., & Maia, B. H. L. (2007). Attraction of fruitâ€eating bats with essential oils of fruits: A potential tool for forest restoration. Biotropica, 39(1), 136-140.
Brinati, A., Oliveira, J. M., Oliveira, V. S., Barros, M. S., Carvalho, B. M., Oliveira, L. S., ... & Freitas, M. B. (2016). Low, chronic exposure to endosulfan induces bioaccumulation and decreased carcass total fatty acids in neotropical fruit bats. Bulletin of Environmental Contamination and Toxicology, 97(5), 626-631.
Buege, J. A., & Aust, S. D. (1978). [30] Microsomal lipid peroxidation. In Methods in enzymology (Vol. 52, pp. 302-310). Academic press.
Chargui, I., Grissa, I., Bensassi, F., Hrira, M. Y., Haouem, S., Haouas, Z., & Bencheikh, H. (2012). Oxidative stress, biochemical and histopathological alterations in the liver and kidney of female rats exposed to low doses of deltamethrin (DM): a molecular assessment. Biomedical and Environmental Sciences, 25(6), 672-683.
Chauhan, L. K., Kumar, M., Paul, B. N., Goel, S. K., & Gupta, S. K. (2007). Cytogenetic effects of commercial formulations of deltamethrin and/or isoproturon on human peripheral lymphocytes and mouse bone marrow cells. Environmental and Molecular Mutagenesis, 48(8), 636-643.
Chionh, Y. T., Cui, J., Koh, J., Mendenhall, I. H., Ng, J. H., Low, D., ... & Wang, L. F. (2019). High basal heat-shock protein expression in bats confers resistance to cellular heat/oxidative stress. Cell Stress and Chaperones, 24(4), 835-849.
Cummins, I., Dixon, D. P., Freitag-Pohl, S., Skipsey, M., & Edwards, R. (2011). Multiple roles for plant glutathione transferases in xenobiotic detoxification. Drug metabolism reviews, 43(2), 266-280.
Cutler, R. G. (1985). Peroxide-producing potential of tissues: inverse correlation with longevity of mammalian species. Proceedings of the National Academy of Sciences, 82(14), 4798-4802.
Davies, T. G. E., Field, L. M., & Williamson, M. S. (2012). The reâ€emergence of the bed bug as a nuisance pest: implications of resistance to the pyrethroid insecticides. Medical and veterinary entomology, 26(3), 241-254.
de Oliveira, J. M., de Almeida Lima, G. D., Destro, A. L. F., Condessa, S., Zuanon, J. A. S., Freitas, M. B., & de Oliveira, L. L. (2021). Short-term intake of deltamethrin-contaminated fruit, even at low concentrations, induces testicular damage in fruit-eating bats (Artibeus lituratus). Chemosphere, 278, 130423.
de Souza, M. B., de Souza Santos, L. R., Borges, R. E., Nunes, H. F., Vieira, T. B., Pacheco, S. M., & de Melo e Silva, D. (2020). Current status of ecotoxicological studies of bats in Brazil. Bulletin of environmental contamination and toxicology, 104(4), 393-399.
de Souza, T. C., da Silva, S. L. R., Marcon, J. L., & Waichman, A. V. (2020). Acute toxicity of deltamethrin to Amazonian freshwater fish. Toxicology and Environmental Health Sciences, 12(2), 149-155.
Desneux, N., Decourtye, A., & Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual review of entomology, 52(1), 81-106.
Dieterich, S., Bieligk, U., Beulich, K., Hasenfuss, G., & Prestle, J. (2000). Gene expression of antioxidative enzymes in the human heart: increased expression of catalase in the end-stage failing heart. Circulation, 101(1), 33-39.
Dietz, S., De Roman, M., Lauck-Birkel, S., Maus, C., Neumann, P., & Fischer, R. (2009). Ecotoxicological and environmental profile of the insecticide delta methrin. In Pyrethroid Scientific Forum 2009 (p. 211).
Dubey, N., Khan, A. M., & Raina, R. (2013). Sub-acute deltamethrin and fluoride toxicity induced hepatic oxidative stress and biochemical alterations in rats. Bulletin of environmental contamination and toxicology, 91(3), 334-338.
Folch, J., Lees, M., & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J biol Chem, 226(1), 497-509.
Freitas, M. B., Passos, C. B., Vasconcelos, R. B., & Pinheiro, E. C. (2005). Effects of short-term fasting on energy reserves of vampire bats (Desmodus rotundus). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 140(1), 59-62.
Freitas, M. B., Welker, A. F., & Pinheiro, E. C. (2006). Efeitos do jejum e da variação sazonal sobre as reservas lipídicas de morcegos hematófagos (Chiroptera: Phyllostomidae). Brazilian Journal of Biology, 66, 1051-1055.
Freitas, R. M., Linhares, B. S., Oliveira, J. M., Leite, J. P. V., da Matta, S. L. P., Gonçalves, R. V., & Freitas, M. B. (2021). Tebuconazole-induced toxicity and the protective effect of Ficus carica extract in Neotropical fruit-eating bats. Chemosphere, 275, 129985.
Frisard, M., & Ravussin, E. (2006). Energy metabolism and oxidative stress. endocrine, 29(1), 27-32.
Froese, J. M., Smits, J. E., Forsyth, D. J., & Wickstrom, M. L. (2009). Toxicity and immune system effects of dietary deltamethrin exposure in tiger salamanders (Ambystoma tigrinum). Journal of Toxicology and Environmental Health, Part A, 72(8), 518-526.
Guardiola, F. A., Gónzalez-Párraga, P., Meseguer, J., Cuesta, A., & Esteban, M. A. (2014). Modulatory effects of deltamethrin-exposure on the immune status, metabolism and oxidative stress in gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology, 36(1), 120-129.
Habig, W. H., Pabst, M. J., & Jakoby, W. B. (1974). Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry, 249(22), 7130-7139.
Hayes, J. D., Flanagan, J. U., & Jowsey, I. R. (2005). Glutathione transferases. Annual review of pharmacology and toxicology, 45(1), 51-88.
Hermes-Lima, M. (2004). Oxygen in biology and biochemistry: role of free radicals. Functional metabolism: regulation and adaptation, 1, 319-66.
Jones, G., Jacobs, D. S., Kunz, T. H., Willig, M. R., & Racey, P. A. (2009). Carpe noctem: the importance of bats as bioindicators. Endangered species research, 8(1-2), 93-115.
Ku, H. H., Brunk, U. T., & Sohal, R. S. (1993). Relationship between mitochondrial superoxide and hydrogen peroxide production and longevity of mammalian species. Free Radical Biology and Medicine, 15(6), 621-627.
Li, M., Liu, X., & Feng, X. (2019). Cardiovascular toxicity and anxiety-like behavior induced by deltamethrin in zebrafish (Danio rerio) larvae. Chemosphere, 219, 155-164.
Liao, C. H., He, X. J., Wang, Z. L., Barron, A. B., Zhang, B., Zeng, Z. J., & Wu, X. B. (2018). Short-term exposure to lambda-cyhalothrin negatively affects the survival and memory-related characteristics of worker bees Apis mellifera. Archives of environmental contamination and toxicology, 75(1), 59-65.
Limón-Pacheco, J., & Gonsebatt, M. E. (2009). The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 674(1-2), 137-147.
Lowry, O. H. (1951). Protein measurement with the Folin phenol reagent. J biol Chem, 193, 265-275.
Lu, Q., Sun, Y., Ares, I., Anadón, A., Martínez, M., Martínez-Larrañaga, M. R., ... & Martínez, M. A. (2019). Deltamethrin toxicity: A review of oxidative stress and metabolism. Environmental research, 170, 260-281.
Lushchak, V. I., & Storey, K. B. (2021). Oxidative stress concept updated: Definitions, classifications, and regulatory pathways implicated. EXCLI journal, 20, 956.
Magalhães, J. P. D., Costa, J., & Church, G. M. (2007). An analysis of the relationship between metabolism, developmental schedules, and longevity using phylogenetic independent contrasts. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 62(2), 149-160.
Martins, M. P. V.; Torres, J. M.; Dos Anjos, E. A. C. (2014). Dieta de morcegos filostomídeos (Mammalia, Chiroptera, (Phyllostomidae) em fragmento urbano do Instituto São Vicente, Campo Grande, Mato Grosso do Sul. Papeis Avulsos de Zoologia, v. 54, n. 20, p. 665-670.
Melo, F. P., Rodriguezâ€Herrera, B., Chazdon, R. L., Medellin, R. A., & Ceballos, G. G. (2009). Small tentâ€roosting bats promote dispersal of largeâ€seeded plants in a Neotropical forest. Biotropica, 41(6), 737-743.
Munshi-South, J., & Wilkinson, G. S. (2010). Bats and birds: exceptional longevity despite high metabolic rates. Ageing research reviews, 9(1), 12-19.
Nunes, H., Rocha, F. L., & Cordeiro-Estrela, P. (2017). Bats in urban areas of Brazil: roosts, food resources and parasites in disturbed environments. Urban ecosystems, 20(4), 953-969.
Oliveira, J. M., Condessa, S. S., Destro, A. L. F., Lima, G. D. A., Cupertino, M. C., Cardoso, S. A., Freitas, M. B., & Oliveira, L. L. (2022). Morphophysiological alterations in fruit-eating bats after oral exposure to deltamethrin. International Journal of Experimental Pathology, 00:1-12.
Oliveira, J. M., Destro, A. L. F., Freitas, M. B., & Oliveira, L. L. (2020). How do pesticides affect bats?-A brief review of recent tions. Brazilian Journal of Biology, 81, 499-507.
Oliveira, J. M., Losano, N. F., Condessa, S. S., de Freitas, R. M. P., Cardoso, S. A., Freitas, M. B., & de Oliveira, L. L. (2018). Exposure to deltamethrin induces oxidative stress and decreases of energy reserve in tissues of the Neotropical fruit-eating bat Artibeus lituratus. Ecotoxicology and environmental safety, 148, 684-692.
Oliveira, J. M., Brinati, A., Miranda, L. D. L., Morais, D. B., Zanuncio, J. C., Gonçalves, R. V., ... & Freitas, M. B. (2017). Exposure to the insecticide endosulfan induces liver morphology alterations and oxidative stress in fruitâ€eating bats (Artibeus lituratus). International Journal of Experimental Pathology, 98(1), 17-25.
Romero, A., Ares, I., Ramos, E., Castellano, V., Martinez, M., Martínez-Larrañaga, M. R., ... & Martínez, M. A. (2015). Evidence for dose-additive effects of a type II pyrethroid mixture. In vitro assessment. Environmental Research, 138, 58-66.
Royauté, R., Buddle, C. M., & Vincent, C. (2015). Under the influence: sublethal exposure to an insecticide affects personality expression in a jumping spider. Functional Ecology, 29(7), 962-970.
Santos, M. A., Rodrigues, M. V., Áreas, M. A., & Reyes, F. G. (2011). Deltamethrin and Permethrin in the liver and heart of Wistar rats submitted to oral subchronic exposure. Journal of the Brazilian Chemical Society, 22, 891-896.
Siwicki, A. K., Terechâ€Majewska, E., Grudniewska, J., Malaczewska, J., Kazun, K., & Lepa, A. (2010). Influence of deltamethrin on nonspecific cellular and humoral defense mechanisms in rainbow trout (Oncorhynchus mykiss). Environmental Toxicology and Chemistry: An International Journal, 29(3), 489-491.
Sjörgren, B., Noerdenskjold, T., Holmgeen, H. And Mollerstrom, J., 1938. Beitrag zur kenntnis der leberrhythmik (glykogen, phosphor und calcium in der kaninchenleber). Pflügers Archiv, p. 240-247.
Stahlschmidt, P., Hahn, M., & Brühl, C. A. (2017). Nocturnal risks-high bat activity in the agricultural landscape indicates potential pesticide exposure. Frontiers in Environmental Science, 5, 62.
Tanikawa, K., & Torimura, T. (2006). Studies on oxidative stress in liver diseases: important future trends in liver research. Medical molecular morphology, 39(1), 22-27.
Torquetti, C. G., Guimarães, A. T. B., & Soto-Blanco, B. (2021). Exposure to pesticides in bats. Science of the Total Environment, 755, 142509.
Vizotto, L. D., & Taddei, V. A. (1973). Chave para determinação de quirópteros brasileiros.
Yang, C., Lim, W., & Song, G. (2020). Mediation of oxidative stress toxicity induced by pyrethroid pesticides in fish. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 234, 108758.
Yildirim, M. Z., Benlı, A. Ç. K., Selvı, M., Özkul, A., Erkoç, F., & Koçak, O. (2006). Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings. Environmental Toxicology: An International Journal, 21(6), 614-620.
Descargas
Publicado
Número
Sección
Licencia
Derechos de autor 2023 Os autores mantêm os direitos autorais de seus artigos sem restrições, concedendo ao editor direitos de ção não exclusivos.
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Os artigos estão licenciados sob uma licença Creative Commons Atribuição-NãoComercial-SemDerivações 4.0 Internacional (CC BY-NC-ND 4.0). O acesso é livre e gratuito para download e leitura, ou seja, é permitido copiar e redistribuir o material em qualquer mídia ou formato.