Genetic Variability of Natural Fragments of Luehea divaricata Mart. & Zucc. in the Atlantic Forest Biome
DOI:
https://doi.org/10.37002/biodiversidadebrasileira.v11i4.1837Keywords:
Genetic erosion, gene flow, germplasm, rehabilitation of degraded areasAbstract
In this study we used microsatellite markers to analyse the genetic variability of three natural fragments of Luehea divaricata Mart. & Zucc. in the Atlantic Forest, aiming to identify their potential for use in rehabilitation of degraded areas. With the GenAlEx v. 6.5 software, genetic variability parameters and its partition between and within fragments were estimated. Higher genetic variability was observed within the analyzed fragments (77%), which is expected for species whose reproduction is predominantly by crosses. A gene flow greater than 1 (Nm= 3.853) was estimated, which is sufficient to homogenize allelic frequencies and make genetically similar fragments. Due to the high gene flow between fragments, the genetic differentiation index was low (FST = 0.072), which makes them less susceptible to the adverse effects of fragmentation. Therefore, we found that there is genetic variability in the studied L. divaricata fragments, and this information is relevant for planning recovery of degraded areas, in which germplasm collections should prioritize the number of individuals per fragment.
References
Carvalho PE. 2008. Açoita-cavalo (Luehea divaricata). Circular Técnica, 147. Colombo, PR. 9p.
Charlesworth D. Effects of inbreeding on the genetic diversity of populations. Philosophical Transactions of the Royal Society B, 358(1434): 1051-1070, 2003.
Conson ARO, et al. Genetic structure of the Atlantic Rainforest tree species Luehea divaricata (Malvaceae). Genetica, 141: 205-215, 2013.
Ferreira ME & Grattapaglia D. 1998. Introdução ao uso de marcadores moleculares em análise genética. 1 ed. Brasília: EMBRAPA-CENARGEN. 220p.
Flôres AV, et al. Estabelecimento e multiplicação in vitro de Luehea divaricata Mart. & Zucc. Ciência Florestal, 21(1): 175-182, 2011.
Frankham R, Briscoe DA & Ballou JD. 2002. Introduction to conservation genetics. Cambridge University Press. 617p.
Gonçalves AR, Chaves LJ & Telles MPC. Genetic variability and effective population size in Hymenaea stigonocarpa (Fabaceae) germplasm collection: tools for breeding programs and genetic conservation. Genetica, 147: 359-368, 2019.
Hartl DL & Clark AG. 2010. Princípios de genética de populações. 4 ed. Porto Alegre: Artmed. 659p.
León EAB. 2014. Qualidade de sementes, micropropagação, conservação in vitro e isolamento de DNA genômico de Luehea divaricata Mart. & Zucc. Tese (Doutorado em Engenharia Florestal). Universidade Federal de Santa Maria. 209p.
Lorenzi H. 2008. Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 1 vol. Nova Odessa: Plantarum. 384p.
Moura TM, Sebbenn AM, Chaves LJ, Coelho ASG, Oliveira GCX & Kageyama PY. Diversidade e estrutura genética espacial em populações fragmentadas de Solanum spp. do Cerrado, estimadas por meio de locos microssatélites. Scientia Forestalis, 37(82): 143-150, 2009.
Nagel JC, et al. Historical gene flow within and among populations of Luehea divaricata in the Brazilian Pampa. Genetica, 143(3): 317-29, 2015.
Nascimento ART, Longhi, SJ & Brena DA. Estrutura e padrões de distribuição espacial de espécies arbóreas em uma amostra de floresta ombrófila mista em Nova Prata, RS. Ciência Florestal, 11(1): 105-119, 2001.
Peakall R & Smouse PE. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28: 2537-2539, 2012.
Reed DH. Relationship between population size and fitness. Conservation Biology, 19(2): 563-568, 2005.
Roesch LFW, et al. The Brazilian Pampa: A Fragile Biome. Diversity, 1: 182-198, 2009.
Ruas EA, et al. Isolation and characterization of ten microsatellite loci for the tree species Luehea divaricata Mart. (Malvaceae) and intergeneric transferability. Conservation Genetics Resources, 1(1): 245, 2009.
Slatkin M. Rare alleles as indicators of gene flow. Evolution, 39(1): 53-65, 1985. Templeton AR. 2011. Genética de Populações e Teoria Microevolutiva. Ribeirão Preto: Sociedade Brasileira de Genética – SBG. 705p.
Souza CG, Zanella L, Borém RAT, Carvalho LMT, Alves HMR & Volpato MML. Análise da fragmentação florestal da área de proteção ambiental Coqueiral, Coqueiral/ MG. Ciência Florestal, 24(3): 631-644, 2014.
Tobler WR. A computer movie simulating urban growth in the Detroit region. Economic Geography, 46(2): 234-240, 1970.
Vogler DW & Kalisz S. Sex among the flowers: the distribution of plant mating systems. Evolution, 55(1): 202-204, 2001.
Wright S. The genetical structure of populations. Annals of Eugenics, 15(1): 323-354, 1951.
Wright S. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution, 19(3): 395-420, 1965.
Zucchi MI. 2002. Análise da estrutura genética Eugenia dysenterica DC utilizando marcadores RAPD e SSR. Tese (Doutorado em Genética e Melhoramento de Plantas). Universidade de São Paulo. 130p.
Downloads
Published
Issue
Section
License
Copyright (c) 2021 Biodiversidade Brasileira - BioBrasil
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Os artigos estão licenciados sob uma licença Creative Commons Atribuição-NãoComercial-SemDerivações 4.0 Internacional (CC BY-NC-ND 4.0). O acesso é livre e gratuito para download e leitura, ou seja, é permitido copiar e redistribuir o material em qualquer mídia ou formato.
v3-3-0-3-2-1-8-release.27